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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using Phospho-Cardiac Troponin I (Ser23/24) Polyclonal a (30937-1-AP)

    WB analysis of mouse heart using 30937-1-AP

    Phosphatase inhibitor treated mouse heart tissue, λ phosphatase treated mouse heart tissue, and non-treated mouse heart tissue were subjected to SDS PAGE followed by western blot with 30937-1-AP (Phospho-TNNI3 (Ser23/24) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Phospho-Cardiac Troponin I (Ser23/24) Polyclonal antibody

Phospho-Cardiac Troponin I (Ser23/24) Polyclonal Antibody for WB, ELISA
Cat No. 30937-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

Cardiac troponin I, CMD2A, CMH7, cTnI, Phospho-Cardiac Troponin I (Ser23/24), RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, λ phosphatase treated mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30937-1-AP targets Phospho-Cardiac Troponin I (Ser23/24) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name troponin I type 3 (cardiac)
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight26 kDa
GenBank accession numberBC096165
Gene Symbol Cardiac Troponin I
Gene ID (NCBI) 7137
RRIDAB_3669787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)

Protocols

Product Specific Protocols
WB protocol for Phospho-Cardiac Troponin I (Ser23/24) antibody 30937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols