Phospho-Cardiac Troponin I (Ser23/24) Polyclonal antibody
Phospho-Cardiac Troponin I (Ser23/24) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 30937-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, λ phosphatase treated mouse heart tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30937-1-AP targets Phospho-Cardiac Troponin I (Ser23/24) in WB, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | troponin I type 3 (cardiac) |
Calculated molecular weight | 210 aa, 24 kDa |
Observed molecular weight | 26 kDa |
GenBank accession number | BC096165 |
Gene symbol | Cardiac Troponin I |
Gene ID (NCBI) | 7137 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-Cardiac Troponin I (Ser23/24) antibody 30937-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |