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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Caspase 9 (Ser196) Recombinant antibody, PBS Only

Phospho-Caspase 9 (Ser196) Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3P16

Cat no : 80346-1-PBS

Synonyms

CASP9, Caspase 9, Caspase 9/p35/p10, Apoptotic protease Mch-6, Apoptotic protease Mch 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Caspase 9 (Ser196) Recombinant antibody (80346-1-RR)

    WB analysis using 80346-1-RR (same clone as 80346-1-PBS)

    Non-treated HEK-293 and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80346-1-RR (Phospho-Caspase 9 (Ser196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 80346-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-Caspase 9 (Ser196) Recombinant antibody (80346-1-RR)

    WB analysis using 80346-1-RR (same clone as 80346-1-PBS)

    Non-treated Jurkat and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80346-1-RR (Phospho-Caspase 9 (Ser196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 80346-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-Caspase 9 (Ser196) Recombinant antibody (80346-1-RR)

    WB analysis using 80346-1-RR (same clone as 80346-1-PBS)

    Non-treated NIH/3T3 and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80346-1-RR (Phospho-Caspase 9 (Ser196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 80346-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-Caspase 9 (Ser196) Recombinant antibody (80346-1-RR)

    IF Staining of HeLa using 80346-1-RR (same clone as 80346-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-Caspase 9 (Ser196) antibody (80346-1-RR, Clone: 3P16 ) at dilution of 1:3075 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 80346-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80346-1-PBS targets Phospho-Caspase 9 (Ser196) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name caspase 9, apoptosis-related cysteine peptidase
Calculated molecular weight 46 kDa
Observed molecular weight46 kDa, 35 kDa
GenBank accession numberBC002452
Gene symbol Caspase 9
Gene ID (NCBI) 842
RRIDAB_2918890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46kDa precursor protein which can be cleaved into a 35kDa subunit and a 11kDa subunit. The phosphorylated type can be detected at 55kDa and 35kDa. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It can be phosphorylated by PKB/AKT1 at Ser196, this modification will downregulate its activity and decrease apoptosis. Akt phosphorylation site found in human caspase 9 is absent in mouse caspase 9.It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507) In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].