Phospho-Chk1 (Ser345) Polyclonal antibody
Phospho-Chk1 (Ser345) Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 28803-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells |
| Positive IF/ICC detected in | Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
28803-1-AP targets Phospho-Chk1 (Ser345) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | CHK1 checkpoint homolog (S. pombe) |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC004202 |
| Gene symbol | Chk1 |
| Gene ID (NCBI) | 1111 |
| RRID | AB_2918206 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Chk1 (Ser345) antibody 28803-1-AP | Download protocol |
| IF protocol for Phospho-Chk1 (Ser345) antibody 28803-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Oncol The ATR inhibitor VE-821 increases the sensitivity of gastric cancer cells to cisplatin | ||
Phytomedicine Epiberberine induced p53/p21-dependent G2/M cell cycle arrest and cell apoptosis in gastric cancer cells by activating γ-aminobutyric acid receptor- β3 | ||
J Exp Clin Cancer Res HIF-1α-HPRT1 axis promotes tumorigenesis and gefitinib resistance by enhancing purine metabolism in EGFR-mutant lung adenocarcinoma |
