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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Cofilin (Ser3) Polyclonal antibody

Phospho-Cofilin (Ser3) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29715-1-AP

Synonyms

18 kDa phosphoprotein, CFL, CFL1, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18, Phospho-Cofilin (Ser3)

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-Cofilin (Ser3) Polyclonal antibody (29715-1-AP)

    WB analysis using 29715-1-AP

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29715-1-AP (Phospho-Cofilin (Ser3) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-Cofilin (Ser3) Polyclonal antibody (29715-1-AP)

    IF Staining of HeLa using 29715-1-AP

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-Cofilin (Ser3) antibody (29715-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inλ phosphatase treated HeLa cells, HeLa cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29715-1-AP targets Phospho-Cofilin (Ser3) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC012318
Gene symbol Cofilin
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for Phospho-Cofilin (Ser3) antibody 29715-1-APDownload protocol
IF protocol for Phospho-Cofilin (Ser3) antibody 29715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Udesh (Verified Customer) (03-05-2024)

Worked well for WB of glioma cells at 1:800 in 3% BSA

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:800
  • Cell Tissue Type: GBM