• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-EGFR (Tyr1092) Polyclonal antibody (30278-1-AP)

    WB analysis using 30278-1-AP

    Non-treated HeLa and EGF treated HeLa cells were subjected to SDS PAGE followed by western blot with 30278-1-AP (Phospho-EGFR (Tyr1092) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Phospho-EGFR (Tyr1092) Polyclonal antibody

Phospho-EGFR (Tyr1092) Polyclonal Antibody for WB, ELISA
Cat No. 30278-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

EGFR, ERBB, ERBB1, HER1, mENA, Phospho-EGFR (Tyr1068), Phospho-EGFR (Tyr1092), PIG61, Proto oncogene c ErbB 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inEGF treated HeLa cells

This antibody, also known as EGFR Tyr1068, is the site where the signal peptide is removed.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30278-1-AP targets Phospho-EGFR (Tyr1092) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight 1210 aa, 134 kDa
Observed molecular weight170 kDa
GenBank accession numberBC094761
Gene Symbol EGFR
Gene ID (NCBI) 1956
RRIDAB_3085350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1092 as EGFR Tyr1068, the site where the signaling peptide is removed.

Protocols

Product Specific Protocols
WB protocol for Phospho-EGFR (Tyr1092) antibody 30278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols