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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-EIF2AK4 (Thr899) Recombinant antibody, PBS Only

Phospho-EIF2AK4 (Thr899) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5N13

Cat no : 82835-3-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-EIF2AK4 (Thr899) Recombinant antibody (82835-3-RR)

    WB analysis using 82835-3-RR (same clone as 82835-3-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82835-3-RR (Phospho-EIF2AK4 (Thr899) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82835-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-EIF2AK4 (Thr899) Recombinant antibody (82835-3-RR)

    WB analysis using 82835-3-RR (same clone as 82835-3-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82835-3-RR (Phospho-EIF2AK4 (Thr899) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82835-3-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82835-3-PBS targets Phospho-EIF2AK4 (Thr899) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2 alpha kinase 4
Calculated molecular weight 187 kDa
Observed molecular weight187 kDa
GenBank accession numberBC009350
Gene symbol EIF2AK4
Gene ID (NCBI) 440275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4) belongs to a family of protein kinases that phosphorylate the α subunit of EIF2 (eukaryotic translation initiation factor 2) in response to various stress stimuli. Phosphorylation of EIF2A in response to EIF2AK4 activation subsequently increases translation of specific mRNAs that are important for stress remediation such as ATF4 (activating transcription factor 4). (PMID: 26986695)