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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-EIF2S1 (Ser51) Monoclonal antibody, PBS Only (Detector)

Phospho-EIF2S1 (Ser51) Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4A11

Cat no : 68023-1-PBS

Synonyms

EIF2S1, EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-EIF2S1 (Ser51) Monoclonal antibody (68023-1-Ig)

    WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-EIF2S1 (Ser51) Monoclonal antibody (68023-1-Ig)

    WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

    Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-EIF2S1 (Ser51) Monoclonal antibody (68023-1-Ig)

    WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (HRP-66009) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-EIF2S1 (Ser51) Monoclonal antibody (68023-1-Ig)

    WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-EIF2S1 (Ser51) Monoclonal antibody (68023-1-Ig)

    WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

    Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Phospho-EIF2S1 (Ser51) Monoclonal antibody (68023-1-Ig)

    WB analysis of HEK-293 using 68023-1-Ig (same clone as 68023-1-PBS)

    Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-EIF2S1 (Ser51) Monoclonal antibody (68023-1-Ig)

    IF Staining of HeLa using 68023-1-Ig (same clone as 68023-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-EIF2S1 (Ser51) antibody (68023-1-Ig, Clone: 1A4A11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using Phospho-EIF2S1 (Ser51) Monoclonal antibody (68023-1-Ig)

    FC experiment of PC-3 using 68023-1-Ig (same clone as 68023-1-PBS)

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-EIF2S1 (Ser51) (68023-1-Ig, Clone:1A4A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP50181-1

    Cytometric bead array in cell lysate using MP50181-1

    Cytometric bead array in cell lysate using MP50181-1, Phospho-EIF2S1 (Ser51) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68479-1-PBS. Detection antibody: 68023-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68023-1-PBS targets Phospho-EIF2S1 (Ser51) as part of a matched antibody pair:

MP50181-1: 68479-1-PBS capture and 68023-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Calculated molecular weight 36 kDa
Observed molecular weight36 kDa
GenBank accession numberNM_004094
Gene symbol EIF2S1
Gene ID (NCBI) 1965
RRIDAB_2918767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.