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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-EIF4B (Ser406) Polyclonal antibody

Phospho-EIF4B (Ser406) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28779-1-AP

Synonyms

EIF4B, PRO1843, eIF-4B, EIF 4B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-EIF4B (Ser406) Polyclonal antibody (28779-1-AP)

    WB analysis using 28779-1-AP

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28779-1-AP (Phospho-EIF4B (Ser406) antibody) at dilution of 1:60000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-EIF4B (Ser406) Polyclonal antibody (28779-1-AP)

    WB analysis using 28779-1-AP

    Non-treated PC-3 cells, phosphatase inhibitor treated PC-3 cells and λ phosphatase treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28779-1-AP (Phospho-EIF4B (Ser406) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-EIF4B (Ser406) Polyclonal antibody (28779-1-AP)

    IF Staining of HeLa using 28779-1-AP

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using Phospho-EIF4B (Ser406) antibody (28779-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inPC-3 cells, λ phosphatase treated PC-3 cells
Positive IF/ICC detected insodium arsenite treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28779-1-AP targets Phospho-EIF4B (Ser406) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4B
Calculated molecular weight 611 aa, 69 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC073154
Gene symbol EIF4B
Gene ID (NCBI) 1975
RRIDAB_2918201
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623). EIF4B Ser406 was identified as a novel phosphorylation site regulated by mitogens, and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.

Protocols

Product Specific Protocols
WB protocol for Phospho-EIF4B (Ser406) antibody 28779-1-APDownload protocol
IF protocol for Phospho-EIF4B (Ser406) antibody 28779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols