Phospho-EIF4B (Ser422) Polyclonal antibody
Phospho-EIF4B (Ser422) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 28781-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Calyculin A treated HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28781-1-AP targets Phospho-EIF4B (Ser422) in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | eukaryotic translation initiation factor 4B |
Calculated molecular weight | 611 aa, 69 kDa |
Observed molecular weight | 70-80 kDa |
GenBank accession number | BC073154 |
Gene symbol | EIF4B |
Gene ID (NCBI) | 1975 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other EIF proteins are EIF4A, EIF4F. EIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of EIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623). Ser422 is regarded as the major modification site of EIF4B that is phosphorylated in response to amino-acid refeeding(PMID: 22750809).
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-EIF4B (Ser422) antibody 28781-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |