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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-EIF4B (Ser422) Polyclonal antibody (28781-1-AP)

    WB analysis using 28781-1-AP

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28781-1-AP (Phospho-EIF4B (Ser422) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Phospho-EIF4B (Ser422) Polyclonal antibody

Phospho-EIF4B (Ser422) Polyclonal Antibody for WB, ELISA
Cat No. 28781-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

EIF 4B, EIF4B, Phospho-EIF4B (Ser422), PRO1843

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inCalyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28781-1-AP targets Phospho-EIF4B (Ser422) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4B
Calculated molecular weight 611 aa, 69 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC073154
Gene Symbol EIF4B
Gene ID (NCBI) 1975
RRIDAB_2935409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other EIF proteins are EIF4A, EIF4F. EIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of EIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623). Ser422 is regarded as the major modification site of EIF4B that is phosphorylated in response to amino-acid refeeding(PMID: 22750809).

Protocols

Product Specific Protocols
WB protocol for Phospho-EIF4B (Ser422) antibody 28781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols