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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibody, PBS Only

Phospho-ERK1/2 (Thr202/Tyr204) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8D12

Cat no : 80031-1-PBS

Synonyms

ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, Phospho-ERK1/2 (Thr202/Tyr204), PRKM3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    WB analysis of HeLa using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (ERK1/2-phospho-Tyr204 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80031-1-PBS targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 3
Calculated molecular weight 38-43 kDa
Observed molecular weight38-40 kDa
GenBank accession numberNM_002746
Gene symbol ERK1
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.