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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    WB analysis of HeLa using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (ERK1/2-phospho-Tyr204 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibod (80031-1-RR)

    FC experiment of HepG2 using 80031-1-RR (same clone as 80031-1-PBS)

    1X10^6 HepG2 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibody (80031-1-RR, Clone:8D12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

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Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibody, PBS Only

Phospho-ERK1/2 (Thr202/Tyr204) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 80031-1-PBS
Clone No.8D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

ERK1/2 (Thr202/Tyr204), p ERK1/2, p ERK1/2 (Thr202/Tyr204), p ERK1/2 Thr202/Tyr204, p-ERK1/2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80031-1-PBS targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 3
Calculated molecular weight 38-43 kDa
Observed molecular weight38-40 kDa
GenBank accession numberNM_002746
Gene Symbol ERK1
Gene ID (NCBI) 5595
RRIDAB_2918858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27361
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.