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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-GRB10 (Ser476) Polyclonal antibody (28873-1-AP)

    WB analysis using 28873-1-AP

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28873-1-AP (Phospho-GRB10 (Ser476) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

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Phospho-GRB10 (Ser476) Polyclonal antibody

Phospho-GRB10 (Ser476) Polyclonal Antibody for WB, ELISA
Cat No. 28873-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Synonyms

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, Phospho-GRB10 (Ser476), RSS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inλ phosphatase treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28873-1-AP targets Phospho-GRB10 (Ser476) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name growth factor receptor-bound protein 10
Calculated molecular weight536aa,61 kDa; 594aa,67 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC024285
Gene Symbol GRB10
Gene ID (NCBI) 2887
RRIDAB_2918210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13322
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the INSR and INS-like growth factor (IGF1R) receptors. It may play a role in mediating INS-stimulated ubiquitination of INSR, leading to proteasomal degradation. It has been reported that GRB10 is a direct substrate of mechanistic/mammalian target of mTOR and can be phosphorylated at Ser150, Ser428 and Ser476 identified as MAPK-mediated phosphorylation sites. (PMID: 15952796, 24746805, 21659605)

Protocols

Product Specific Protocols
WB protocol for Phospho-GRB10 (Ser476) antibody 28873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols