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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-GSK3B (Ser9) Monoclonal antibody, PBS Only

Phospho-GSK3B (Ser9) Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C9E2

Cat no : 67558-1-PBS

Synonyms

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, GSK3B S9, GSK3B Ser9, gsk3beta, Phospho-GSK3B (Ser9), Ser9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-GSK3B (Ser9) Monoclonal antibody (67558-1-Ig)

    WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)

    Non-treated and Calyculin A treated cell lysates were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-GSK3B (Ser9) Monoclonal antibody (67558-1-Ig)

    WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)

    Non-treated and Calyculin A treated PC-3 were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-GSK3B (Ser9) Monoclonal antibody (67558-1-Ig)

    WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)

    Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Phospho-GSK3B (Ser9) Monoclonal antibody (67558-1-Ig)

    IHC staining of human liver cancer using 67558-1-Ig (same clone as 67558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67558-1-Ig (GSK3B-phospho-S9 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Phospho-GSK3B (Ser9) Monoclonal antibody (67558-1-Ig)

    IHC staining of human liver cancer using 67558-1-Ig (same clone as 67558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67558-1-Ig (GSK3B-phospho-S9 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using Phospho-GSK3B (Ser9) Monoclonal antibody (67558-1-Ig)

    IF Staining of PC-3 using 67558-1-Ig (same clone as 67558-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Calyculin A treated PC-3 cells and non-treated PC-3 cells using Phospho-GSK3B (Ser9) antibody (67558-1-Ig, Clone: 1C9E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67558-1-PBS targets Phospho-GSK3B (Ser9) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name glycogen synthase kinase 3 beta
Observed molecular weight 48 kDa
GenBank accession numberNM_002093
Gene symbol GSK3B
Gene ID (NCBI) 2932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .