Validation Data Gallery
Tested Applications
| Positive WB detected in | TNF-alpha treated HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
28900-1-AP targets Phospho-HSP27 (Ser78) in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 27kDa protein 1 |
| Calculated molecular weight | 19-23 kDa |
| Observed molecular weight | 27-30 kDa |
| GenBank accession number | BC012768 |
| Gene Symbol | HSPB1 |
| Gene ID (NCBI) | 3315 |
| RRID | AB_2881230 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04792 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-HSP27 (Ser78) antibody 28900-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
JOR Spine Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and bone marrow stem cells from patients with adolescent idiopathic scoliosis |