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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-HSP27 (Ser78) Polyclonal antibody

Phospho-HSP27 (Ser78) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28900-1-AP

Synonyms

DMPK binding protein, heat shock 27kDa protein 2, Heat shock protein beta 2, Hs.78846, Hsp B1, HSP27, Phospho-HSP27 (Ser78)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-HSP27 (Ser78) Polyclonal antibody (28900-1-AP)

    WB analysis using 28900-1-AP

    Non-treated HeLa and TNF alpha (HZ-1014) treated HeLa cells were subjected to SDS PAGE followed by western blot with 28900-1-AP (Phospho-HSP27 (Ser78) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-HSP27 (Ser78) Polyclonal antibody (28900-1-AP)

    WB analysis using 28900-1-AP

    Non-treated and Calyculin A treated HeLa cells and PC-3 cells were subjected to SDS PAGE followed by western blot with 28900-1-AP (Phospho-HSP27 (Ser78) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

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Tested Applications

Positive WB detected inTNF-alpha treated HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28900-1-AP targets Phospho-HSP27 (Ser78) in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name heat shock 27kDa protein 1
Calculated molecular weight 19-23 kDa
Observed molecular weight27-30 kDa
GenBank accession numberBC012768
Gene symbol HSPB1
Gene ID (NCBI) 3315
RRIDAB_2881230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)

Protocols

Product Specific Protocols
WB protocol for Phospho-HSP27 (Ser78) antibody 28900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

JOR Spine

Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and bone marrow stem cells from patients with adolescent idiopathic scoliosis

Authors - Sihan He
View Article