Phospho-Histone H2A.X (Ser139) Polyclonal antibody

Phospho-Histone H2A.X (Ser139) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29380-1-AP

Synonyms

H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x, Phospho-Histone H2A.X (Ser140), γH2AX



Tested Applications

Positive WB detected inUV treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Product Information

29380-1-AP targets Phospho-Histone H2A.X (Ser139) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name H2A histone family, member X
Calculated molecular weight 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC013416
Gene symbol H2AFX
Gene ID (NCBI) 3014
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser140 (a form called cH2AX) by the kinases ATM and ATR and is progressively dephosphorylated at Tyr143 by the Eya1 and Eya3 tyrosine phosphatases, resulting in a temporal switch from a postulated diphosphorylated (pSer140, pTyr143) to monophosphorylated (pSer140) H2AX state. Because phosphorylation of H2AX at Ser 140 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA damage produced and the subsequent repair of the DNA lesion. The phosphorylation site of H2AX, Ser140, has also been described as Ser139 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 17615256, PMID:22941631)

Protocols

Product Specific Protocols
WB protocol for Phospho-Histone H2A.X (Ser139) antibody 29380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols