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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-INPPL1 (Tyr986/987) Polyclonal antibody (29574-1-AP)

    WB analysis using 29574-1-AP

    Non-treated and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

  • Western Blot (WB) analysis of various lysates using Phospho-INPPL1 (Tyr986/987) Polyclonal antibody (29574-1-AP)

    WB analysis using 29574-1-AP

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

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Phospho-INPPL1 (Tyr986/987) Polyclonal antibody

Phospho-INPPL1 (Tyr986/987) Polyclonal Antibody for WB, ELISA
Cat No. 29574-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

INPPL 1, INPPL1, Phospho-INPPL1 (Tyr986/987), Protein 51C, SHIP 2, SHIP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inUV treated HeLa cells, UV treated NIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29574-1-AP targets Phospho-INPPL1 (Tyr986/987) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate phosphatase-like 1
Calculated molecular weight 1258 aa, 139 kDa
Observed molecular weight150 kDa
GenBank accession numberBC140853
Gene Symbol INPPL1
Gene ID (NCBI) 3636
RRIDAB_3085347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15357
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Inositol polyphosphate phosphatase-like protein 1(INPPL1) belongs to the inositol and phosphatidylinositol 5-phosphatase family, also known as SH2 domain-containing inositol 5'-phosphatase 2(SHIP2), is ubiquitous in all organizations. SHIP2 is constitutively tyrosine-phosphorylated in chronic myeloid leukemia progenitor cells. In B cells, SHIP2 was most tyrosine phosphorylated after BCR and FC-γriib crosslinking and was associated with Shc, but not after BCR stimulation alone. It has also been shown that SHIP2 can be phosphorylated at Tyr-986 in COS-7 cells and Chinese hamster ovary cells overexpressing the insulin receptor. (PMID: 11349134)

Protocols

Product Specific Protocols
WB protocol for Phospho-INPPL1 (Tyr986/987) antibody 29574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols