Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
60666-1-PBS targets Phospho-JNK (Thr183/Tyr185) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase 8 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 42 kDa, 50 kDa |
| GenBank accession number | NM_138982 |
| Gene Symbol | JNK |
| Gene ID (NCBI) | 5599 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P45983 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.