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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR)

    WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR)

    WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

    Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR)

    WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

    Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR)

    IF Staining of HEK-293 using 80024-1-RR (same clone as 80024-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed UV treated HEK-293 cells using Phospho-JNK (Tyr185) antibody (80024-1-RR, Clone: 2G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR)

    FC experiment of HEK-293 using 80024-1-RR (same clone as 80024-1-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR, Clone:2G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

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Phospho-JNK (Tyr185) Recombinant antibody, PBS Only

Phospho-JNK (Tyr185) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 80024-1-PBS
Clone No.2G8
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

JNK (Tyr185), p JNK , p JNK (Tyr185), p JNK Tyr185, Phospho JNK

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80024-1-PBS targets Phospho-JNK (Tyr185) in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 8
Observed molecular weight37-46 kDa
GenBank accession numberNM_139049
Gene Symbol JNK
Gene ID (NCBI) 5599
RRIDAB_2882943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45983
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)

Publications

SpeciesApplicationTitle
human,ratWB,IHC

Redox Rep

Selenium participates in the formation of kidney stones by alleviating endoplasmic reticulum stress and apoptosis of renal tubular epithelial cells

Authors - Xiaozhe Su
View Article