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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-JNK (Tyr185) Recombinant antibody, PBS Only

Phospho-JNK (Tyr185) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G8

Cat no : 80024-1-PBS

Synonyms

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, Phospho-JNK (Tyr185), PRKM8, SAPK1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR)

    WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR)

    WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR)

    WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80024-1-PBS targets Phospho-JNK (Tyr185) in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 8
Observed molecular weight37-46 kDa
GenBank accession numberNM_139049
Gene symbol JNK
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)