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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-JUN (Ser63) Recombinant antibody (84147-1-RR)

    WB analysis using 84147-1-RR

    Non-treated NIH/3T3 cells, Anisomycin treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84147-1-RR (Phospho-JUN (Ser63) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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Phospho-JUN (Ser63) Recombinant antibody

Phospho-JUN (Ser63) Recombinant Antibody for WB, ELISA
Cat No. 84147-1-RR
Clone No.240965B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

AP1, AP 1, JUN, c Jun, Activator protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inAnisomycin treated NIH/3T3 cells, UV treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84147-1-RR targets Phospho-JUN (Ser63) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name jun oncogene
Calculated molecular weight 331 aa, 36 kDa
Observed molecular weight40-48 kDa
GenBank accession numberBC068522
Gene Symbol JUN
Gene ID (NCBI) 3725
RRIDAB_3671710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05412
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)

Protocols

Product Specific Protocols
WB protocol for Phospho-JUN (Ser63) antibody 84147-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols