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Phospho-MAP2K4 (Ser257/Thr261) Polyclonal antibody

Phospho-MAP2K4 (Ser257/Thr261) Polyclonal Antibody for WB, ELISA
Cat No. 29250-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, MEK4, MKK4, Phospho-MAP2K4, Phospho-MAP2K4 (Ser257/Thr261), P-MAP2K4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inPDGF treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

29250-1-AP targets Phospho-MAP2K4 (Ser257/Thr261) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 4
Calculated molecular weight 399 aa, 44 kDa
Observed molecular weight44-50 kDa
GenBank accession numberBC036032
Gene Symbol MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2918258
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP45985
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitogen-activated protein kinase kinase 4 (MAP2K4), also known as MAP kinase kinase 4 (MKK4), is a second-level kinase in the hierarchical mitogen-activated protein kinase (MAPK) pathway and is regulated by Ras. MAP2K4 has been found to be stably mutated in 3-5 % of cancers, such as ovarian, prostate, and liver cancers. MAP2K4 is activated by MEKK1 or ASK1/TAK1/MLK3. It is unique among all known MAP2Ks in its ability to phosphorylate two different MAPKs, p38 and JNK (c-Jun N-terminal kinase), respectively. (PMID: 24487067, PMID: 33341545)

Protocols

Product Specific Protocols
WB protocol for Phospho-MAP2K4 (Ser257/Thr261) antibody 29250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Phytomedicine

Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity

Authors - Zhong Xian
humanWB

J Inorg Biochem

Approaches to selective and potent inhibition of glioblastoma by vanadyl complexes: Inducing mitotic catastrophe and methuosis

Authors - Sha Xu
mouseWB

Bioorg Med Chem

Synthesis and biological evaluation of lappaconitine analogues as potential anti-neuroinflammatory agents by side chain modification and scaffold hopping strategy

Authors - Feng Xing
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