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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-MCL1 (Thr163) Polyclonal antibody

Phospho-MCL1 (Thr163) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29560-1-AP

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-MCL1 (Thr163) Polyclonal antibody (29560-1-AP)

    WB analysis using 29560-1-AP

    Non-treated and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 29560-1-AP (Phospho-MCL1 (Thr163) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

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Tested Applications

Positive WB detected inMG132 treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29560-1-AP targets Phospho-MCL1 (Thr163) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Calculated molecular weight 350 aa, 37 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC017197
Gene symbol MCL1
Gene ID (NCBI) 4170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCL1 is an anti-apoptotic member of the BCL-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Recent studies show that MCL1 is upregulated in numerous hematological and solid tumor malignancies. Therefore, MCL1 has been suggested as a potential new therapeutic target. MCL1 can be phosphorylated by several protein kinases which enables the recognition of MCL1 by its E3 ubiquitin-ligases TrCP or FBW7 (PMID: 33308268). MCL1 shows higher stability when phosphorylated on threonine 163 (PMID: 16543145).

Protocols

Product Specific Protocols
WB protocol for Phospho-MCL1 (Thr163) antibody 29560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols