Phospho-MEK1 (Ser298) Monoclonal antibody
Phospho-MEK1 (Ser298) Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3F10G10
Cat no : 68047-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, nocodazole treated A431 cells, Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
68047-1-Ig targets Phospho-MEK1 (Ser298) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase kinase 1 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 40-50 kDa |
| GenBank accession number | BC139729 |
| Gene symbol | MEK1 |
| Gene ID (NCBI) | 5604 |
| RRID | AB_2918789 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MEK1 (Ser298) antibody 68047-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Mol Med Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer | ||
ACS Appl Mater Interfaces Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels. | ||
Cell Rep Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism |
