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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-MEK1 (Thr386) Polyclonal antibody

Phospho-MEK1 (Thr386) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28935-1-AP

Synonyms

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MKK1, Phospho-MEK1 (Thr386), PRKMK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-MEK1 (Thr386) Polyclonal antibody (28935-1-AP)

    WB analysis using 28935-1-AP

    Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 28935-1-AP (Phospho-MEK1 (Thr386) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inλ phosphatase treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28935-1-AP targets Phospho-MEK1 (Thr386) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 1
Calculated molecular weight 43 kDa
Observed molecular weight40-45 kDa
GenBank accession numberBC139729
Gene symbol MEK1
Gene ID (NCBI) 5604
RRIDAB_2918218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis. MEK1 has 2 isoforms produced by alternative splicing with the molecular weight of 43 kDa and 41 kDa. MEK1 may form a heterodimer with MEK2.(PMID:21144847,19219045).

Protocols

Product Specific Protocols
WB protocol for Phospho-MEK1 (Thr386) antibody 28935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols