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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-MEK1 (Thr386) Monoclonal antibody, PBS Only (Detector)

Phospho-MEK1 (Thr386) Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6A2

Cat no : 68015-1-PBS

Synonyms

MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis using 68015-1-Ig (same clone as 68015-1-PBS)

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis of HeLa using 68015-1-Ig (same clone as 68015-1-PBS)

    Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis using 68015-1-Ig (same clone as 68015-1-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis using 68015-1-Ig (same clone as 68015-1-PBS)

    Non-treated HEK-293 cells and nocodazole treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-MEK1 (Thr386) Monoclonal antibody (68015-1-Ig)

    WB analysis using 68015-1-Ig (same clone as 68015-1-PBS)

    Non-treated A431 cells and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP50180-1

    Cytometric bead array in cell lysate using MP50180-1

    Cytometric bead array in cell lysate using MP50180-1, Phospho-MEK1 (Thr386) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67872-1-PBS. Detection antibody: 68015-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68015-1-PBS targets Phospho-MEK1 (Thr386) as part of a matched antibody pair:

MP50180-1: 67872-1-PBS capture and 68015-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 1
Calculated molecular weight 43 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC139729
Gene symbol MEK1
Gene ID (NCBI) 5604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)