Phospho-MEK1 (Thr386) Monoclonal antibody, PBS Only (Detector)
Phospho-MEK1 (Thr386) Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
1G6A2
Cat no : 68015-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
68015-1-PBS targets Phospho-MEK1 (Thr386) as part of a matched antibody pair:
MP50180-1: 67872-1-PBS capture and 68015-1-PBS detection (validated in N/A)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | mitogen-activated protein kinase kinase 1 |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 40-50 kDa |
GenBank accession number | BC139729 |
Gene symbol | MEK1 |
Gene ID (NCBI) | 5604 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)