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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-MEK6 (Ser207) Polyclonal antibody (28903-1-AP)

    WB analysis using 28903-1-AP

    Non-treated HEK-293 cells, UV treated HEK-293 cells, non-treated HeLa cells and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 28903-1-AP (Phospho-MEK6 (Ser207) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Phospho-MEK6 (Ser207) Polyclonal antibody

Phospho-MEK6 (Ser207) Polyclonal Antibody for WB, ELISA
Cat No. 28903-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, Phospho-MAP2K6 (Ser207), Phospho-MEK3 (Ser218)/MEK6 (Ser207), Phospho-MEK6 (Ser207), PRKMK6, SAPKK3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inUV treated HEK-293 cells, HEK-293 cells, HeLa cells, Anisomycin treated HeLa cells

The antibody also detects the phosphorylated at Ser218 of the MEK3.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28903-1-AP targets Phospho-MEK6 (Ser207) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 6
Calculated molecular weight 334 aa, 37 kDa
Observed molecular weight35-37 kDa
GenBank accession numberBC012009
Gene Symbol MEK6
Gene ID (NCBI) 5608
RRIDAB_2935411
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52564
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. Phosphorylation of Ser207 and Thr211 by MAP3Ks is critical for MEK6 activation, and acetylation of these resides by VopA blocks MEK6 activity(PMID: 26648936). The antibody also recognizes the ser218 phosphorylation site of MEK3.

Protocols

Product Specific Protocols
WB protocol for Phospho-MEK6 (Ser207) antibody 28903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols