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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-MLKL (Ser358) Recombinant antibody

Phospho-MLKL (Ser358) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241469D2

Cat no : 82090-2-RR

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using Phospho-MLKL (Ser358) Recombinant antibody (82090-2-RR)

    WB analysis of HT-29 using 82090-2-RR

    Non-treated HT-29 cells, TNF alpha and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 82090-2-RR (Phospho-MLKL (Ser358) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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Tested Applications

Positive WB detected inTNF alpha and Calyculin A treated HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82090-2-RR targets Phospho-MLKL (Ser358) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mixed lineage kinase domain-like
Calculated molecular weight 54 kDa
Observed molecular weight54 kDa
GenBank accession numberXM_005255834
Gene symbol MLKL
Gene ID (NCBI) 197259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily. One common method for inducing necroptosis is by treating cells grown in culture with a mixture composed of TNF-α, a Smac-mimetic, that degrades cellular inhibitors of apoptosis (cIAPs) and the pancaspase inhibitor Z-VAD-FMK. This mixture is abbreviated as T/S/Z. It drives necrosome complex formation and RIPK3-dependent phosphorylation of Thr-357 and Ser-358 located within the C-terminal kinase-like domain of human MLKL. (PMID: 28878015)

Protocols

Product Specific Protocols
WB protocol for Phospho-MLKL (Ser358) antibody 82090-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols