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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using Phospho-MNK1 (Thr250/255) Recombinant antibody (81398-1-RR)

    WB analysis of HEK-293T using 81398-1-RR (same clone as 81398-1-PBS)

    Non-treated HEK-293T cells, phosphatase inhibitor and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81398-1-RR (Phospho-MNK1 (Thr250/255) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Phospho-MNK1 (Thr250/255) Recombinant antibody (81398-1-RR)

    WB analysis of HEK-293 using 81398-1-RR (same clone as 81398-1-PBS)

    WB result of Phospho-MNK1 (Thr250/255) antibody (81398-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-MNK1 (Thr250/255) transfected HEK-293 cells. This data was developed using the same antibody clone with 81398-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Phospho-MNK1 (Thr250/255) Recombinant antibody, PBS Only

Phospho-MNK1 (Thr250/255) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 81398-1-PBS
Clone No.4P9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

MKNK1, Mnk1, MAPK signal-integrating kinase 1, MAP kinase-interacting serine/threonine-protein kinase 1, MAP kinase signal-integrating kinase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

This antibody is equivalent to Thr197/202 in mice.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81398-1-PBS targets Phospho-MNK1 (Thr250/255) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name MAP kinase interacting serine/threonine kinase 1
Calculated molecular weight 51 kDa
Observed molecular weight51 kDa
GenBank accession numberBC002755
Gene Symbol MNK1
Gene ID (NCBI) 8569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BUB5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MNK1/MKNK1 (MAPK Interacting Serine/Threonine Kinase 1) is a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. MNK1 regulates diverse biologic processes including translation, cell proliferation, and differentiation through phosphorylation of different substrates(PMID: 29045529). Through phosphorylation MNK1 regulates the function of eukaryotic translation initiation factor 4E (eIF4E), a key player in translational control, whose expression is mostly upregulated in cancer patients.