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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-MOBKL1B (Thr12) Polyclonal antibody

Phospho-MOBKL1B (Thr12) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29027-1-AP

Synonyms

C2orf6, FLJ10788, FLJ11595, MATS1, MOB1, Mob1 alpha, Mob1 homolog 1B, Mob1A, MOB1B, Mob4B, MOBK1B, MOBKL1B, Phospho-MOB1B (Thr12), Phospho-MOBKL1B (Thr12), Protein Mob4B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-MOBKL1B (Thr12) Polyclonal antibody (29027-1-AP)

    WB analysis using 29027-1-AP

    Phosphatase inhibitor treated and λ phosphatase treated PC-12 cells were subjected to SDS PAGE followed by western blot with 29027-1-AP (Phospho-MOBKL1B (Thr12) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

Positive WB detected inPC-12 cells, λ phosphatase treated PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29027-1-AP targets Phospho-MOBKL1B (Thr12) in WB, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name MOB1, Mps One Binder kinase activator-like 1B (yeast)
Calculated molecular weight 216 aa, 25 kDa
Observed molecular weight25 kDa
GenBank accession numberBC003398
Gene symbol MOBKL1B
Gene ID (NCBI) 55233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. MOB1 protein is a key regulator of large tumor suppressor 1/2 (LATS1/2) kinases in the Hippo pathway. MOBKL1A and MOBKL1B are phosphorylated by MST1/MST2 kinases at Thr35 and Thr12, and MST1/MST2-catalyzed phosphorylation of MOBKL1A/MOBKL1B in intact cells is sufficient to substantially retard cell-cycle progression (PMID: 18328708).

Protocols

Product Specific Protocols
WB protocol for Phospho-MOBKL1B (Thr12) antibody 29027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Cell Physiol

m6 A-induced TRIB3 regulates Hippo pathway through interacting with LATS1 to promote the progression of lung adenocarcinoma

Authors - Jiamei Wu
View Article