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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-MYL9 (Thr19/Ser20) Recombinant antibody

Phospho-MYL9 (Thr19/Ser20) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G10

Cat no : 82717-1-RR

Synonyms

MYL9, MLC-2C, MLC2, 1G10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-MYL9 (Thr19/Ser20) Recombinant antibody (82717-1-RR)

    WB analysis using 82717-1-RR

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-MYL9 (Thr19/Ser20) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-MYL9 (Thr19/Ser20) Recombinant antibody (82717-1-RR)

    WB analysis using 82717-1-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-MYL9 (Thr19/Ser20) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-MYL9 (Thr19/Ser20) Recombinant antibody (82717-1-RR)

    FC experiment of HeLa using 82717-1-RR

    1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-MYL9 (Thr19/Ser20) Recombinant antibody (82717-1-RR, Clone:1G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

Positive WB detected inCalyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82717-1-RR targets Phospho-MYL9 (Thr19/Ser20) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 9, regulatory
Calculated molecular weight 20 kDa
Observed molecular weight19-20 kDa
GenBank accession numberBC002648
Gene symbol MYL9
Gene ID (NCBI) 10398
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).

Protocols

Product Specific Protocols
WB protocol for Phospho-MYL9 (Thr19/Ser20) antibody 82717-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols