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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-NDRG1 (Thr346) Polyclonal antibody (30886-1-AP)

    WB analysis using 30886-1-AP

    Non-treated A431 cells, phosphatase inhibitor treated A431 cells and λ phosphatase treated A431 cells were subjected to SDS PAGE followed by western blot with 30886-1-AP (Phospho-NDRG1 (Thr346) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Phospho-NDRG1 (Thr346) Polyclonal antibody

Phospho-NDRG1 (Thr346) Polyclonal Antibody for WB, ELISA
Cat No. 30886-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

CAP43, CMT4D, DRG 1, DRG1, GC4, HMSNL, N myc downstream regulated 1, NDR1, NDRG1, Protein NDRG1, PROXY1, RIT42, RTP, TARG1, TDD5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, λ phosphatase treated A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30886-1-AP targets Phospho-NDRG1 (Thr346) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name N-myc downstream regulated 1
Calculated molecular weight 43 kDa
Observed molecular weight48 kDa
GenBank accession numberBC006260
Gene Symbol NDRG1
Gene ID (NCBI) 10397
RRIDAB_3669774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

N-Myc Downstream Regulated 1 (NDRG1) is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. The phosphorylation of NDRG1 (on Thr346) showed the greatest statistically significant increase in clear cell renal cell carcinoma (ccRCC) compared with noncancerous renal tissue. Further study showed the phosphorylation of NDRG1 (Thr346) in ccRCC cells was maintained by RICTOR and mTOR in complex 2. (PMID: 37298315)

Protocols

Product Specific Protocols
WB protocol for Phospho-NDRG1 (Thr346) antibody 30886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols