Phospho-NF-κB p65 (Ser468) Recombinant antibody
Phospho-NF-κB p65 (Ser468) Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse and More (4)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
6N1
Cat no : 82335-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 25 publications below |
| IF | See 1 publications below |
Product Information
82335-1-RR targets Phospho-NF-κB p65 (Ser468) in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, rat, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | BC011603 |
| Gene symbol | RELA |
| Gene ID (NCBI) | 5970 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-NF-κB p65 (Ser468) antibody 82335-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice | ||
Adv Healthc Mater Hyaluronic Acid-based ROS-responsive Multifunctional Injectable Hydrogel Platform Accelerating Diabetic Wound Healing | ||
Int J Biol Macromol Angelica sinensis aboveground part polysaccharide and its metabolite 5-MT ameliorate colitis via modulating gut microbiota and TLR4/MyD88/NF-κB pathway | ||
PLoS Pathog Negative regulation of type I interferon signaling by integrin-linked kinase permits dengue virus replication | ||
Int Immunopharmacol Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis | ||
J Ethnopharmacol ZeXieYin formula alleviates atherosclerosis by inhibiting the MAPK/NF-κB signaling pathway in APOE-/- mice to attenuate vascular inflammation and increase plaque stability |
