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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-NF-κB p65 (Ser536) Recombinant antibody (80379-2-RR)

    WB analysis using 80379-2-RR (same clone as 80379-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80379-2-RR (Phospho-NF-κB p65 (Ser536) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80379-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-NF-κB p65 (Ser536) Recombinant antibody (80379-2-RR)

    WB analysis of NIH/3T3 using 80379-2-RR (same clone as 80379-2-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80379-2-RR (Phospho-NF-κB p65 (Ser536) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80379-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using Phospho-NF-κB p65 (Ser536) Recombinant antibody (80379-2-RR)

    FC experiment of PC-3 using 80379-2-RR (same clone as 80379-2-PBS)

    1x10^6 untreated or Calyculin A treated PC-3 cells were intracellularly stained with 0.25 ug Phospho-NF-κB p65 (Ser536) Recombinant antibody (80379-2-RR, Clone:240777D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue), 1x10^6 untreated PC-3 cells were intracellularly stained with 0.25 ug Phospho-NF-κB p65 (Ser536) Recombinant antibody (80379-2-RR, Clone:240777D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(black). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80379-2-PBS in a different storage buffer formulation.

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Phospho-NF-κB p65 (Ser536) Recombinant antibody, PBS Only

Phospho-NF-κB p65 (Ser536) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80379-2-PBS
Clone No.240777D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

NF κB, NF-κB p65, p65, p65; RELA, RELA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80379-2-PBS targets Phospho-NF-κB p65 (Ser536) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated molecular weight 65 kDa
Observed molecular weight75 kDa
GenBank accession numberBC011603
Gene Symbol NF-κB p65
Gene ID (NCBI) 5970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ04206
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.