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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-NF2 (Ser518) Polyclonal antibody (28851-1-AP)

    WB analysis using 28851-1-AP

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 28851-1-AP (Phospho-NF2 (Ser518) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Phospho-Neurofibromin 2 (Ser518) Polyclonal antibody

Phospho-Neurofibromin 2 (Ser518) Polyclonal Antibody for WB, ELISA
Cat No. 28851-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

Phospho-NF2 (Ser518), NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin 2, Neurofibromin-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCalyculin A treated HEK-293T cells, Calyculin A treated PC-3 cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28851-1-AP targets Phospho-Neurofibromin 2 (Ser518) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name neurofibromin 2 (merlin)
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007279
Gene Symbol NF2
Gene ID (NCBI) 4771
RRIDAB_2935410
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors. 28851-1-AP antibody is specific to phosphorylation of NF2 at serine 518. (PMID: 11782491, 14724586, 18361411, 18835652, 32115406)

Protocols

Product Specific Protocols
WB protocol for Phospho-Neurofibromin 2 (Ser518) antibody 28851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols