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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TrKA (Tyr496) Recombinant antibody (84976-1-RR)

    WB analysis using 84976-1-RR

    Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 84976-1-RR (Phospho-NTRK1 (Tyr496) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

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Phospho-TrkA (Tyr496)/TrkB (Tyr516) Recombinant antibody

Phospho-TrkA (Tyr496)/TrkB (Tyr516) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84976-1-RR
Clone No.241917B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho-NTRK1, NTRK1 (Tyr496), Phospho-NTRK1 (Tyr496), Phospho-NTRK1-Tyr496, Phospho-TrkA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpervanadate treated HeLa cells

This antibody recognizes TrkA-II (isoform 1) phosphorylation at Tyr496 or TrkA-I (isoform 2) phosphorylation at Tyr490.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84976-1-RR targets Phospho-TrkA (Tyr496)/TrkB (Tyr516) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name neurotrophic tyrosine kinase, receptor, type 1
Observed molecular weight140 kDa
GenBank accession numberBC062580
Gene Symbol NTRK1
Gene ID (NCBI) 4914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The tyrosine kinase receptor TrkA (or neurotrophin receptor kinase 1, NTRK1) has been predominately studied for its role during the development of the nervous system. In developing neurons, TrkA activation upon binding of its ligands nerve growth factor (NGF) or neurotrophin-3 (NT-3) results in the stimulation of various tyrosine kinase-induced signaling pathways leading to neuronal outgrowth. (PMID: 32957504) This antibody recognizes the phosphorylation of TrkA at site 496, which is the phosphorylation of the TrkA-I(isoform 2) sequence at site 490.

Protocols

Product Specific Protocols
WB protocol for Phospho-TrkA (Tyr496)/TrkB (Tyr516) antibody 84976-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols