Phospho-P53 (Ser15) Polyclonal antibody
Phospho-P53 (Ser15) Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 28961-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | etoposide treated HT-29 cells |
Positive IF/ICC detected in | etoposide treated HT-29 cells, HT-29 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 24 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
28961-1-AP targets Phospho-P53 (Ser15) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | tumor protein p53 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 53 kDa |
GenBank accession number | BC003596 |
Gene symbol | P53 |
Gene ID (NCBI) | 7157 |
RRID | AB_2881236 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-P53 (Ser15) antibody 28961-1-AP | Download protocol |
IF protocol for Phospho-P53 (Ser15) antibody 28961-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation | ||
Acta Pharmacol Sin Exosomes are involved in total body irradiation-induced intestinal injury in mice. | ||
J Nutr Biochem Lycopene suppresses gastric cancer cell growth without affecting normal gastric epithelial cells | ||
Clin Epigenetics Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line | ||
Apoptosis High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines. | ||
J Biol Chem Vibrio splendidus flagellin C binds tropomodulin to induce p38 MAPK-mediated p53-dependent coelomocyte apoptosis in Echinodermata. |