Phospho-PDH E1 Alpha (Ser232) Polyclonal antibody

Phospho-PDH E1 Alpha (Ser232) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29582-1-AP

Synonyms

PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PHE1A, Phospho-PDH E1 Alpha (Ser232)


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Tested Applications

Positive WB detected inHeLa cells, Serum-free treated HeLa cells, 10 % fetal bovine serum treated HeLa cells
Positive IF/ICC detected inλ phosphatase treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29582-1-AP targets Phospho-PDH E1 Alpha (Ser232) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Calculated molecular weight 43 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC002406
Gene symbol PDHA1
Gene ID (NCBI) 5160
RRIDAB_2918327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

Protocols

Product Specific Protocols
WB protocol for Phospho-PDH E1 Alpha (Ser232) antibody 29582-1-APDownload protocol
IF protocol for Phospho-PDH E1 Alpha (Ser232) antibody 29582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cardiovasc Med

MCC950, a Selective NLRP3 Inhibitor, Attenuates Adverse Cardiac Remodeling Following Heart Failure Through Improving the Cardiometabolic Dysfunction in Obese Mice.

Authors - Menglong Wang
humanWB

FASEB J

PDK4 facilitates fibroblast functions and diabetic wound healing through regulation of HIF-1α protein stability and gene expression

Authors - Zhouji Ma
human,mouseWB

Biochim Biophys Acta Mol Basis Dis

Iron promotes glycolysis to drive colon tumorigenesis

Authors - Zhaoli Liu
mouseIF

Cell Death Discov

PDK4 rescues high-glucose-induced senescent fibroblasts and promotes diabetic wound healing through enhancing glycolysis and regulating YAP and JNK pathway

Authors - Zhouji Ma