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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using Phospho-PDH E1 Alpha (Ser232) Recombinant antibody (81491-1-RR)

    WB analysis of HEK-293T using 81491-1-RR (same clone as 81491-1-PBS)

    Non-treated HEK-293T cells, phosphatase inhibitor treated and λ phosphatase HEK-293T cells were subjected to SDS PAGE followed by western blot with 81491-1-RR (Phospho-PDH E1 Alpha (Ser232) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81491-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using Phospho-PDH E1 Alpha (Ser232) Recombinant antibody (81491-1-RR)

    FC experiment of HEK-293T using 81491-1-RR (same clone as 81491-1-PBS)

    1X10^6 HEK-293T cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-PDH E1 Alpha (Ser232) Recombinant antibody (81491-1-RR, Clone:1L22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81491-1-PBS in a different storage buffer formulation.

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Phospho-PDH E1 Alpha (Ser232) Recombinant antibody, PBS Only

Phospho-PDH E1 Alpha (Ser232) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81491-1-PBS
Clone No.1L22

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

p PDH E1 Alpha Ser232, p PDH E1 Alpha (Ser232), p PDH E1 Alpha, PDH E1 Alpha (Ser232), Phospho PDH E1 Alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81491-1-PBS targets Phospho-PDH E1 Alpha (Ser232) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Calculated molecular weight 43 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08559
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)