Validation Data Gallery
Tested Applications
| Positive WB detected in | Serum-free treated HeLa cells, 10 % fetal bovine serum treated HeLa cells |
| Positive IF/ICC detected in | λ phosphatase treated HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
29583-1-AP targets Phospho-PDH E1 Alpha (Ser300) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | pyruvate dehydrogenase (lipoamide) alpha 1 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC002406 |
| Gene Symbol | PDHA1 |
| Gene ID (NCBI) | 5160 |
| RRID | AB_2935419 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08559 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PDH E1 Alpha (Ser300) antibody 29583-1-AP | Download protocol |
| IF protocol for Phospho-PDH E1 Alpha (Ser300) antibody 29583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes | ||
Science Boosting neuronal activity-driven mitochondrial DNA transcription improves cognition in aged mice |