Phospho-PDH E1 Alpha (Ser300) Recombinant antibody, PBS Only

Phospho-PDH E1 Alpha (Ser300) Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E19

Cat no : 80572-1-PBS

Synonyms

PDH E1α, PDH E1 Alpha, PDHA1, PDHA 1, PDHA


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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80572-1-PBS targets Phospho-PDH E1 Alpha (Ser300) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Calculated molecular weight 43 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC002406
Gene symbol PDHA1
Gene ID (NCBI) 5160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)