Validation Data Gallery
Tested Applications
| Positive WB detected in | PDGF treated PC-3 cells, PDGF treated NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
29241-1-AP targets Phospho-PDPK1 (Ser241) in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | 3-phosphoinositide dependent protein kinase-1 |
| Calculated molecular weight | 556 aa, 63 kDa |
| Observed molecular weight | 60-63 kDa |
| GenBank accession number | BC012103 |
| Gene Symbol | PDPK1 |
| Gene ID (NCBI) | 5170 |
| RRID | AB_2918256 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15530 |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PDPK1 (Ser241) antibody 29241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism |