Phospho-PERK/EIF2AK3 (Ser719) Polyclonal antibody
Phospho-PERK/EIF2AK3 (Ser719) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (4)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 29546-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | λ phosphatase treated HeLa cells, Thapsigargin treated HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 13 publications below |
Product Information
29546-1-AP targets Phospho-PERK/EIF2AK3 (Ser719) in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | eukaryotic translation initiation factor 2-alpha kinase 3 |
Calculated molecular weight | 1116 aa, 125 kDa |
Observed molecular weight | 140 kDa |
GenBank accession number | BC126354 |
Gene symbol | PERK |
Gene ID (NCBI) | 9451 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 29546-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mar Drugs Ascomylactam C Induces an Immunogenic Cell Death Signature via Mitochondria-Associated ER Stress in Lung Cancer and Melanoma | ||
World J Gastroenterol Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress | ||
Prostaglandins Other Lipid Mediat Dahuang Huanglian Xiexin Decoction ameliorates obesity via modulating adipocyte differentiation and lipid degradation through inhibiting endoplasmic reticulum stress | ||
Tissue Cell XBP1s gene of endoplasmic reticulum stress enhances proliferation and osteogenesis of human periodontal ligament cells | ||
Fish Shellfish Immunol Zinc deficiency causes oxidative stress, endoplasmic reticulum stress, apoptosis and inflammation in hepatocytes in grass carp | ||
Cell Death Dis PDIA4 confers resistance to ferroptosis via induction of ATF4/SLC7A11 in renal cell carcinoma |