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Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody

Phospho-PERK/EIF2AK3 (Thr982) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82534-1-RR
Clone No.4E16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

p PERK/EIF2AK3, p PERK/EIF2AK3 (Thr982), p PERK/EIF2AK3 Thr982, PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inCalyculin A treated HEK-293 cells, NIH/3T3 cells, Calyculin A treated NIH/3T3 cells
Positive FC (Intra) detected inCalyculin A treated HEK-293 cells

This antibody is equivalent to Thr980 in mice.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:11200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

82534-1-RR targets Phospho-PERK/EIF2AK3 (Thr982) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated molecular weight 1116 aa, 125 kDa
Observed molecular weight180 kDa
GenBank accession numberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
RRIDAB_3085359
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

Protocols

Product Specific Protocols
WB protocol for Phospho-PERK/EIF2AK3 (Thr982) antibody 82534-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Vet Microbiol

Bovine parainfluenza virus type 3 infections induce ER stress-mediated autophagy to facilitate virus replication

Authors - Yu Han
humanWB

Cell Death Dis

Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib

Authors - Yu Feng
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