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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PKC Delta (Thr507) Polyclonal antibody (29564-1-AP)

    WB analysis using 29564-1-AP

    Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC Delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently.

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Phospho-PKC Delta (Thr507) Polyclonal antibody

Phospho-PKC Delta (Thr507) Polyclonal Antibody for WB, ELISA
Cat No. 29564-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho-PKC Delta (Thr505), Phospho-PKC Delta, Phospho-PKCδ, Phospho-PKCδ (Thr505), Phospho-PKCδ (Thr507)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPMA treated THP-1 cells, THP-1 cells

This antibody is equivalent to Thr505 in mice.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29564-1-AP targets Phospho-PKC Delta (Thr507) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein kinase C, delta
Calculated molecular weight 78 kDa
Observed molecular weight70 kDa
GenBank accession numberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
RRIDAB_3085346
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKC Delta(PRKCD) is a member of the PKC family, a phospholipid-dependent family of serine/threonine kinases, and is present in human neutrophils. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-507 (activation loop of the kinase domain), Ser-645 (turn motif) and Ser-664 (hydrophobic region), need to be phosphorylated for its full activation. This antibody is equivalent to Thr505 in mice.

Protocols

Product Specific Protocols
WB protocol for Phospho-PKC Delta (Thr507) antibody 29564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols