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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-PKN1 (Thr774) Polyclonal antibody

Phospho-PKN1 (Thr774) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29107-1-AP

Synonyms

DBK, PAK 1, PAK1, Phospho-PKN1 (Thr774), PKN, PKN ALPHA, PKN1, PRK1, PRKCL1, Protease activated kinase 1, Protein kinase C like 1, Protein kinase C like PKN, protein kinase N1, Protein kinase PKN alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PKN1 (Thr774) Polyclonal antibody (29107-1-AP)

    WB analysis using 29107-1-AP

    Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29107-1-AP (Phospho-PKN1 (Thr774) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours.

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Tested Applications

Positive WB detected inPDGF treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29107-1-AP targets Phospho-PKN1 (Thr774) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein kinase N1
Calculated molecular weight 942 aa, 104 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC040061
Gene symbol PKN1
Gene ID (NCBI) 5585
RRIDAB_2918235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)

Protocols

Product Specific Protocols
WB protocol for Phospho-PKN1 (Thr774) antibody 29107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols