Phospho-PLA2G4A (Ser505) Polyclonal antibody
Phospho-PLA2G4A (Ser505) Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 28925-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | λ phosphatase treated HeLa cells |
Positive IF/ICC detected in | λ phosphatase treated HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28925-1-AP targets Phospho-PLA2G4A (Ser505) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | phospholipase A2, group IVA (cytosolic, calcium-dependent) |
Calculated molecular weight | 749 aa, 85 kDa |
Observed molecular weight | 100-110 kDa |
GenBank accession number | BC114340 |
Gene symbol | PLA2G4A |
Gene ID (NCBI) | 5321 |
RRID | AB_2918213 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and and 0.05%BSA. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788)
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-AP | Download protocol |
IF protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |