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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PLA2G4A (Ser505) Polyclonal antibody (28925-1-AP)

    WB analysis using 28925-1-AP

    Non-treated HeLa and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 28925-1-AP (Phospo-PLA2G4A (Ser505) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-PLA2G4A (Ser505) Polyclonal antibody (28925-1-AP)

    IF Staining of HeLa using 28925-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PLA2G4A (Ser505) antibody (28925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Phospho-PLA2G4A (Ser505) Polyclonal antibody

Phospho-PLA2G4A (Ser505) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28925-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, Phospho-PLA2G4A (Ser505), PLA2G4, PLA2G4A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inλ phosphatase treated HeLa cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28925-1-AP targets Phospho-PLA2G4A (Ser505) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Calculated molecular weight 749 aa, 85 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
RRIDAB_2918213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47712
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and and 0.05%BSA.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788)

Protocols

Product Specific Protocols
WB protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-APDownload protocol
IF protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols