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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using Phospho-PLCG2 (Tyr1217) Polyclonal antibody (29568-1-AP)

    WB analysis of Raji using 29568-1-AP

    Non-treated Raji cells and pervanadate treated Raji cells were subjected to SDS PAGE followed by western blot with 29568-1-AP (Phospho-PLCG2 (Tyr1217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

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Phospho-PLCG2 (Tyr1217) Polyclonal antibody

Phospho-PLCG2 (Tyr1217) Polyclonal Antibody for WB, ELISA
Cat No. 29568-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

Phospholipase C gamma 2, Phospholipase C IV, Phospho-PLCG2 (Tyr1217), PLC gamma 2, PLC IV, PLCG2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpervanadate treated Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29568-1-AP targets Phospho-PLCG2 (Tyr1217) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phospholipase C, gamma 2 (phosphatidylinositol-specific)
Calculated molecular weight 148 kDa
Observed molecular weight148-150 kDa
GenBank accession numberBC007565
Gene Symbol PLCG2
Gene ID (NCBI) 5336
RRIDAB_3669689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16885
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)

Protocols

Product Specific Protocols
WB protocol for Phospho-PLCG2 (Tyr1217) antibody 29568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols