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Phospho-POLR2A (Ser2) Polyclonal antibody

Phospho-POLR2A (Ser2) Polyclonal Antibody for WB, ELISA
Cat No. 30888-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inC2C12 cells, λ phosphatase treated C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30888-1-AP targets Phospho-POLR2A (Ser2) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Calculated molecular weight 1970 aa, 217 kDa
Observed molecular weight250 kDa
GenBank accession numberBC137231
Gene Symbol POLR2A
Gene ID (NCBI) 5430
RRIDAB_3669776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)

Protocols

Product Specific Protocols
WB protocol for Phospho-POLR2A (Ser2) antibody 30888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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