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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-PPP1CA (Thr320) Polyclonal antibody

Phospho-PPP1CA (Thr320) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29874-1-AP

Synonyms

Phospho-PPP1CA (Thr320), PP 1A, PPP1A, PPP1CA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PPP1CA (Thr320) Polyclonal antibody (29874-1-AP)

    WB analysis using 29874-1-AP

    Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 29874-1-AP (Phospho-PPP1CA (Thr320) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

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Tested Applications

Positive WB detected inCalyculin A treated HeLa cells, Calyculin A treated Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29874-1-AP targets Phospho-PPP1CA (Thr320) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, catalytic subunit, alpha isoform
Calculated molecular weight 330 aa, 38 kDa
Observed molecular weight37 kDa
GenBank accession numberBC001888
Gene symbol PPP1CA
Gene ID (NCBI) 5499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).

Protocols

Product Specific Protocols
WB protocol for Phospho-PPP1CA (Thr320) antibody 29874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols