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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-RCC1 (Ser11) Polyclonal antibody (14444-1-AP)

    WB analysis using 14444-1-AP

    Non-treated and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 14444-1-AP (Phospho-RCC1 (Ser11) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RCC1 antibody (22142-1-AP) subsequently.

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Phospho-RCC1 (Ser11) Polyclonal antibody

Phospho-RCC1 (Ser11) Polyclonal Antibody for WB, ELISA
Cat No. 14444-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Cell cycle regulatory protein, CHC1, Phospho-RCC1 (Ser11), RCC1, RCC1 I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14444-1-AP targets Phospho-RCC1 (Ser11) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name regulator of chromosome condensation 1
Calculated molecular weight 48 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_001048194
Gene Symbol RCC1
Gene ID (NCBI) 1104
RRIDAB_2179583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against human phospho-CHC1(Ser11) antigen.

Protocols

Product Specific Protocols
WB protocol for Phospho-RCC1 (Ser11) antibody 14444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols