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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-RIPK1 (Ser161) Monoclonal antibody, PBS Only

Phospho-RIPK1 (Ser161) Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2G1

Cat no : 66854-1-PBS

Synonyms

Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161), Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-RIPK1 (Ser161) Monoclonal antibody (66854-1-Ig)

    WB analysis using 66854-1-Ig (same clone as 66854-1-PBS)

    Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-RIPK1 (Ser161) Monoclonal antibody (66854-1-Ig)

    WB analysis using 66854-1-Ig (same clone as 66854-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was untreated (left) or treated with Lambda Protein Phosphatase (λ-PPase, 500U, right) at 37℃ for 1 hours before blocking. This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Phospho-RIPK1 (Ser161) Monoclonal antibody (66854-1-Ig)

    IHC staining of human small intestine using 66854-1-Ig (same clone as 66854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Phospho-RIPK1 (Ser161) Monoclonal antibody (66854-1-Ig)

    IHC staining of human small intestine using 66854-1-Ig (same clone as 66854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Phospho-RIPK1 (Ser161) Monoclonal antibody (66854-1-Ig)

    IHC staining of human heart using 66854-1-Ig (same clone as 66854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Phospho-RIPK1 (Ser161) Monoclonal antibody (66854-1-Ig)

    IHC staining of human heart using 66854-1-Ig (same clone as 66854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Phospho-RIPK1 (Ser161) Monoclonal antibody (66854-1-Ig)

    IF Staining of U2OS using 66854-1-Ig (same clone as 66854-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Phospho-RIPK1 (Ser161) antibody (66854-1-Ig, Clone: 1B2G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66854-1-PBS targets Phospho-RIPK1 (Ser161) in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Calculated molecular weight 76 kDa
Observed molecular weight 74 kDa, 45 kDa
GenBank accession numberNM_003804
Gene symbol RIPK1
Gene ID (NCBI) 8737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).