Phospho-RPS6KA1 (Ser380) Polyclonal antibody
Phospho-RPS6KA1 (Ser380) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 28983-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
28983-1-AP targets Phospho-RPS6KA1 (Ser380) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 1 |
| Calculated molecular weight | 735 aa, 83 kDa |
| Observed molecular weight | 83-90 kDa |
| GenBank accession number | BC014966 |
| Gene symbol | RPS6KA1 |
| Gene ID (NCBI) | 6195 |
| RRID | AB_2881237 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RPS6KA1 (Ser380) antibody 28983-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Increased Nuclear Transporter Importin 7 Contributes to the Tumor Growth and Correlates With CD8 T Cell Infiltration in Cervical Cancer | ||
Cell Death Dis Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration |
